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The cells had been repeatedly cultured in finish medium for 48 h, after which the Beckman Coulter MoFlo Astrios Cell Sorter was utilised to pick out cells with CXCR4-unfavorable expression. These selected cells had been cultured in total medium supplemented with 1 μg/mL of puromycin.I've a number of pictures of the conversion on my aged Computer

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